File(s) stored somewhere else
Please note: Linked content is NOT stored on La Trobe and we can't guarantee its availability, quality, security or accept any liability.
Immune modulation by design: Using topography to control human monocyte attachment and macrophage diﬀerentiation
journal contributionposted on 11.11.2020, 00:49 by David Winkler, Matt Vassey, D Scurr, Grazziela Figueredo, MR Alexander, S Vermeulen, AS Vasilevich, Jan de Boer, Aurelie Carlier, N Beijers, Amir Ghaemmaghami
© 2020 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Macrophages play a central role in orchestrating immune responses to foreign materials, which are often responsible for the failure of implanted medical devices. Material topography is known to influence macrophage attachment and phenotype, providing opportunities for the rational design of “immune-instructive” topographies to modulate macrophage function and thus foreign body responses to biomaterials. However, no generalizable understanding of the inter-relationship between topography and cell response exists. A high throughput screening approach is therefore utilized to investigate the relationship between topography and human monocyte–derived macrophage attachment and phenotype, using a diverse library of 2176 micropatterns generated by an algorithm. This reveals that micropillars 5–10 µm in diameter play a dominant role in driving macrophage attachment compared to the many other topographies screened, an observation that aligns with studies of the interaction of macrophages with particles. Combining the pillar size with the micropillar density is found to be key in modulation of cell phenotype from pro to anti-inflammatory states. Machine learning is used to successfully build a model that correlates cell attachment and phenotype with a selection of descriptors, illustrating that materials can potentially be designed to modulate inflammatory responses for future applications in the fight against foreign body rejection of medical devices.
The EPSRC are gratefully acknowledged for the Next Generation Biomaterials Discovery Programme Grant Funding (Grant no. EP/N006615/1) and the Strategic Equipment grant `3D OrbiSIMS: Label free chemical imaging of materials, cells and tissues' funding that supported this work (grant no. EP/P029868/1). S.V. was supported by the European Union's Horizon 2020 Programme (H2020-MSCA-ITN-2015; Grant agreement 676338). AC kindly acknowledges the Dutch province of Limburg in the LINK (FCL67723) ("Limburg INvesteert in haar Kenniseconomie") knowledge economy project and a VENI grant (number 15075) from the Dutch Science Foundation (NWO). The authors gratefully acknowledge Dr. Chris Gell (University of Nottingham, SLiM) for technical assistance in microscopy as well as Dr Emily F Smith and Dr Craig Stopiello at the Nanoscale and Microscale Research Centre (NMRC University of Nottingham) for acquiring the XPS spectra.
EPSRC | EP/N006615/1
EPSRC | EP/P029868/1
European Union | 676338
Dutch province of Limburg in the LINK ("Limburg INvesteert in haar Kenniseconomie") | FCL67723
Dutch Science Foundation (NWO) | 15075
Rights StatementThe Author reserves all moral rights over the deposited text and must be credited if any re-use occurs. Documents deposited in OPAL are the Open Access versions of outputs published elsewhere. Changes resulting from the publishing process may therefore not be reflected in this document. The final published version may be obtained via the publisher’s DOI. Please note that additional copyright and access restrictions may apply to the published version.
Science & TechnologyPhysical SciencesTechnologyChemistry, MultidisciplinaryNanoscience & NanotechnologyMaterials Science, MultidisciplinaryChemistryScience & Technology - Other TopicsMaterials Sciencebiomaterialshigh-throughput screeningimmune-modulationtopographyFOREIGN-BODY RESPONSESURFACE-CHEMISTRYBIOMATERIALSSHAPEPHAGOCYTOSISIMPLANTSLIBRARYRODENTSBIOLOGYIMPACThigh‐throughput screeningimmune‐modulation