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Hyperosmotic Infusion and Oxidized Surfaces Are Essential for Biofilm Formation of Staphylococcus capitis From the Neonatal Intensive Care Unit
journal contributionposted on 2021-01-22, 05:22 authored by Y Qu, Y Li, DR Cameron, CD Easton, X Zhu, M Zhu, M Salwiczek, Benjamin MuirBenjamin Muir, H Thissen, A Daley, JS Forsythe, AY Peleg, T Lithgow
© Copyright © 2020 Qu, Li, Cameron, Easton, Zhu, Zhu, Salwiczek, Muir, Thissen, Daley, Forsythe, Peleg and Lithgow. Staphylococcus capitis is an opportunistic pathogen often implicated in bloodstream infections in the neonatal intensive care unit (NICU). This is assisted by its ability to form biofilms on indwelling central venous catheters (CVC), which are highly resistant to antibiotics and the immune system. We sought to understand the fundamentals of biofilm formation by S. capitis in the NICU, using seventeen clinical isolates including the endemic NRCS-A clone and assessing nine commercial and two modified polystyrene surfaces. S. capitis clinical isolates from the NICU initiated biofilm formation only in response to hyperosmotic conditions, followed by a developmental progression driven by icaADBC expression to establish mature biofilms, with polysaccharide being their major extracellular polymer substance (EPS) matrix component. Physicochemical features of the biomaterial surface, and in particular the level of the element oxygen present on the surface, significantly influenced biofilm development of S. capitis. A lack of highly oxidized carbon species on the surface prevented the immobilization of S. capitis EPS and the formation of mature biofilms. This information provides guidance in regard to the preparation of hyperosmolar total parenteral nutrition and the engineering of CVC surfaces that can minimize the risk of catheter-related bloodstream infections caused by S. capitis in the NICU.
This work was supported by the National Natural Science Foundation of China (Grant No. 81772241 to YQ) and Micro@Monash seeding Grant to YQ and YL. TL was an ARC Australian Laureate Fellow.
JournalFrontiers in Microbiology
Rights StatementThe Author reserves all moral rights over the deposited text and must be credited if any re-use occurs. Documents deposited in OPAL are the Open Access versions of outputs published elsewhere. Changes resulting from the publishing process may therefore not be reflected in this document. The final published version may be obtained via the publisher’s DOI. Please note that additional copyright and access restrictions may apply to the published version.
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Science & TechnologyLife Sciences & BiomedicineMicrobiologyStaphylococcus capitisbiofilmsbloodstream infectionsNICUcentral venous catheterssurface chemistryoxidized surfacesCOAGULASE-NEGATIVE STAPHYLOCOCCIBLOOD-STREAM INFECTIONSCENTRAL VENOUS CATHETERSBIRTH-WEIGHT INFANTSRESISTANTSTRAINSCLONEDISSEMINATIONMARKERSASSAY