High-throughput sequencing of plasma MicroRNA in chronic fatigue syndrome/myalgic encephalomyelitis
journal contributionposted on 2021-01-07, 05:24 authored by EW Brenu, KJ Ashton, Jana Batovska, DR Staines, SM Marshall-Gradisnik
© 2014 Brenu et al. Background: MicroRNAs (miRNAs) are known to regulate many biological processes and their dysregulation has been associated with a variety of diseases including Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME). The recent discovery of stable and reproducible miRNA in plasma has raised the possibility that circulating miRNAs may serve as novel diagnostic markers. The objective of this study was to determine the role of plasma miRNA in CFS/ME.Results: Using Illumina high-throughput sequencing we identified 19 miRNAs that were differentially expressed in the plasma of CFS/ME patients in comparison to non-fatigued controls. Following RT-qPCR analysis, we were able to confirm the significant up-regulation of three miRNAs (hsa-miR-127-3p, hsa-miR-142-5p and hsa-miR-143-3p) in the CFS/ME patients.Conclusion: Our study is the first to identify circulating miRNAs from CFS/ME patients and also to confirm three differentially expressed circulating miRNAs in CFS/ME patients, providing a basis for further study to find useful CFS/ME biomarkers.
Mason Foundation | 43120
Alison Hunter Memorial Foundation | HF 201
Article NumberARTN e102783
Pagination8p. (p. 1-8)
PublisherPublic Library of Science
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Science & TechnologyMultidisciplinary SciencesScience & Technology - Other TopicsBLOOD MONONUCLEAR-CELLSDIFFERENTIAL EXPRESSIONCIRCULATING MICRORNASPOTENTIAL BIOMARKERSGENE-EXPRESSIONDOWN-REGULATIONCANCERIMMUNERNAAPOPTOSISHumansFatigue Syndrome, ChronicMicroRNAsGene Expression RegulationUp-RegulationAdultMiddle AgedHigh-Throughput Nucleotide SequencingGeneral Science & Technology