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High-throughput phenotyping using digital and hyperspectral imaging-derived biomarkers for genotypic nitrogen response

journal contribution
posted on 08.11.2021, 03:23 by Bikram P Banerjee, Sameer Joshi, Emily Thoday-Kennedy, Raj K Pasam, Josquin Tibbits, Matthew HaydenMatthew Hayden, German SpangenbergGerman Spangenberg, Surya KantSurya Kant
A correction has been published: Journal of Experimental Botany, Volume 72, Issue 13, 22 June 2021, Page 5093, https://doi.org/10.1093/jxb/erab126
Abstract: The development of crop varieties with higher nitrogen use efficiency is crucial for sustainable crop production. Combining high-throughput genotyping and phenotyping will expedite the discovery of novel alleles for breeding crop varieties with higher nitrogen use efficiency. Digital and hyperspectral imaging techniques can efficiently evaluate the growth, biophysical, and biochemical performance of plant populations by quantifying canopy reflectance response. Here, these techniques were used to derive automated phenotyping of indicator biomarkers, biomass and chlorophyll levels, corresponding to different nitrogen levels. A detailed description of digital and hyperspectral imaging and the associated challenges and required considerations are provided, with application to delineate the nitrogen response in wheat. Computational approaches for spectrum calibration and rectification, plant area detection, and derivation of vegetation index analysis are presented. We developed a novel vegetation index with higher precision to estimate chlorophyll levels, underpinned by an image-processing algorithm that effectively removed background spectra. Digital shoot biomass and growth parameters were derived, enabling the efficient phenotyping of wheat plants at the vegetative stage, obviating the need for phenotyping until maturity. Overall, our results suggest value in the integration of high-throughput digital and spectral phenomics for rapid screening of large wheat populations for nitrogen response.

History

Publication Date

25/07/2020

Journal

Journal of Experimental Botany

Volume

71

Issue

15

Pagination

12p. (4604-4615)

Publisher

Oxford University Press

ISSN

0022-0957

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The Author reserves all moral rights over the deposited text and must be credited if any re-use occurs. Documents deposited in OPAL are the Open Access versions of outputs published elsewhere. Changes resulting from the publishing process may therefore not be reflected in this document. The final published version may be obtained via the publisher’s DOI. Please note that additional copyright and access restrictions may apply to the published version.

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