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High-Throughput Quantitation of Cannabinoids by Liquid Chromatography Triple-Quadrupole Mass Spectrometry
journal contributionposted on 2022-07-29, 00:22 authored by Jonathan TranJonathan Tran, AC Elkins, German SpangenbergGerman Spangenberg, Simone RochfortSimone Rochfort
The high-throughput quantitation of cannabinoids is important for the cannabis industry. As medicinal products increase, and research into compounds that have pharmacological benefits increase, and the need to quantitate more than just the main cannabinoids becomes more important. This study aims to provide a rapid, high-throughput method for cannabinoid quantitation using a liquid chromatography triple-quadrupole mass spectrometer (LC-QQQ-MS) with an ultraviolet diode array detector (UV-DAD) for 16 cannabinoids: CBDVA, CBDV, CBDA, CBGA, CBG, CBD, THCV, THCVA, CBN, CBNA, THC, ∆8-THC, CBL, CBC, THCA-A and CBCA. Linearity, limit of detection (LOD), limit of quantitation (LOQ), accuracy, precision, recovery and matrix effect were all evaluated. The validated method was used to determine the cannabinoid concentration of four different Cannabis sativa strains and a low THC strain, all of which have different cannabinoid profiles. All cannabinoids eluted within five minutes with a total analysis time of eight minutes, including column re-equilibration. This was twice as fast as published LC-QQQ-MS methods mentioned in the literature, whilst also covering a wide range of cannabinoid compounds.
This research was funded by Agriculture Victoria Research.
Article NumberARTN 742
Rights Statement© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Science & TechnologyLife Sciences & BiomedicinePhysical SciencesBiochemistry & Molecular BiologyChemistry, MultidisciplinaryChemistrycannabiscannabinoidsLC-QQQ-MSquantitationhigh-throughput quantitationanalysisPRODUCTSQUANTIFICATIONPLANTSCannabinoidsCannabisChromatography, High Pressure LiquidChromatography, LiquidHigh-Throughput Screening AssaysLimit of DetectionPlant ExtractsReproducibility of ResultsSensitivity and SpecificityTandem Mass SpectrometryOrganic ChemistryAnalytical Chemistry not elsewhere classifiedAnalytical Biochemistry