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Helicobacter pylori Growth Stage Determines the Size, Protein Composition, and Preferential Cargo Packaging of Outer Membrane Vesicles
journal contributionposted on 2021-03-15, 05:25 authored by Lauren ZavanLauren Zavan, Natalie BittoNatalie Bitto, Ella JohnstonElla Johnston, David GreeningDavid Greening, Maria LiaskosMaria Liaskos
© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Gram-negative bacteria release outer membrane vesicles (OMVs) as part of their normal growth that contain a range of cargo from their parent bacterium, including DNA, RNA, and proteins. The protein content of OMVs is suggested to be similar in composition to various sub-cellular locations of their parent bacterium. However, very little is known regarding the effect of bacterial growth stage on the size, content, and selective packaging of proteins into OMVs. In this study, the global proteome of Helicobacter pylori and their OMVs throughout bacterial growth are examined to determine if bacterial growth stage affected OMV cargo composition. Analysis of OMVs produced by H. pylori reveals that bacterial growth stage affects the size, composition, and selection of protein cargo into OMVs. Proteomic analysis identifies that the proteome of H. pylori OMVs is vastly different throughout bacterial growth and that OMVs contain a range of proteins compared to their parent bacteria. In addition, bacterial growth stage affects the ability of OMVs to induce the production of IL-8 by human epithelial cells. Therefore, the findings identify that the size, proteome, and immunogenicity of OMVs produced during various stages of bacterial growth is not comparable. Collectively, these findings highlight the importance of considering the bacterial growth stage from which OMVs are isolated, as this will impact their size, protein composition, and ultimately their biological functions.
D. W. G. andM. K-L. contributed equally to this work. This project was supported by a La Trobe University RFA Understanding Diseases Grant (M.K-L), and in part, by the La Trobe University Stone Fellowship (D. W. G), the Australian National Health and Medical Research Council (Project: M. K-L 1107800, D.W.G 1139489,1141964), and funding support from La Trobe University (M. K-L, D.W.G). This work was supported by the LIMS BioImaging Facility (La Trobe University), and the Comprehensive Proteomics Platform (La Trobe University). M. K-L. is a veski Inspiring Women Fellow. L.Z is the recipient of an Australian Society for Microbiology Summer Student Research Award.
Issue1-2 Special Issue: Extracellular Vesicles and Exosomes
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Science & TechnologyLife Sciences & BiomedicineBiochemical Research MethodsBiochemistry & Molecular Biologygrowth stageHelicobacter pyloriIL-8outer membrane vesiclesproteomicssizeGRAM-NEGATIVE BACTERIAPSEUDOMONAS-AERUGINOSAFUNCTIONAL-CHARACTERIZATIONACINETOBACTER-BAUMANNIIPERIPLASMIC PROTEINSBIOGENESISRESISTANCESECRETIONINFECTIONMUTANTSGram-Negative BacteriaBacterial Outer Membrane ProteinsProteomeProteomicsExtracellular Vesicles