Generation of seven iPSC lines from peripheral blood mononuclear cells suitable to investigate Autism Spectrum Disorder
journal contributionposted on 2022-03-29, 03:14 authored by K Bozaoglu, Y Gao, E Stanley, M Fanjul-Fernández, NJ Brown, K Pope, Cherie GreenCherie Green, K Vlahos, K Sourris, M Bahlo, M Delatycki, I Scheffer, PJ Lockhart
We have generated and characterized seven human induced pluripotent stem cell (iPSC) lines derived from peripheral blood mononuclear cells (PBMCs) from a single family, including unaffected and affected individuals clinically diagnosed with Autism Spectrum Disorder (ASD). The reprogramming of the PBMCs was performed using non-integrative Sendai virus containing the reprogramming factors POU5F1 (OCT4), SOX2, KLF4 and MYC. All iPSC lines exhibited a normal karyotype and pluripotency was validated by immunofluorescence, flow cytometry and their ability to differentiate into the three embryonic germ layers. These iPSC lines are a valuable resource to study the molecular mechanisms underlying ASD.
We would like to thank the participants and their families for participating in our research. This study was funded in part by the Australian National Health and Medical Research Council (NHMRC) project grants (GNT1044175 and GNT1098255) awarded to E.G.S, M.B.D, I.S and P.J.L. K.B is supported by an E.H. Flack Fellowship and P.J.L is supported by the Vincent Chiodo Foundation. Additional infrastructure funding to the Murdoch Children's Research Institute was provided by the Australian Government NHMRC Independent Research Institute Infrastructure Support Scheme and the Victorian Government's Operational Infrastructure Support Program. The MCRI iPSC Core Facility is supported by the Stafford Fox Medical Research Foundation. M.B and E.G.S are Research Fellows, and I.S is a Practitioner Fellow, of the NHMRC.
JournalStem Cell Research
Rights Statement© 2019 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/).
Science & TechnologyLife Sciences & BiomedicineCell & Tissue EngineeringBiotechnology & Applied MicrobiologyCell BiologyAdultAutism Spectrum DisorderCellular ReprogrammingFemaleFlow CytometryHumansInduced Pluripotent Stem CellsKruppel-Like Factor 4Kruppel-Like Transcription FactorsLeukocytes, MononuclearMaleOctamer Transcription Factor-3SOXB1 Transcription FactorsSendai virusYoung AdultDevelopmental Biology