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Fungi associated with the ambrosia beetle Xyleborus perforans (Coleoptera: Curculionidae: Scolytinae) on drought-stressed Pinus in New South Wales, Australia

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posted on 2024-02-28, 05:18 authored by ZI Mahony, K Scarlett, AJ Carnegie, Conrad TrollipConrad Trollip, M Laurence, DI Guest
Ambrosia beetles have co-evolved symbiotic relationships with an array of fungal partners. Mutualistic fungal partners are often highly successful in vertical transmission between beetle generations. These persisting relationships can alter beetle behaviour, resulting in the opportunity to occupy new ecological niches and to spread geographically. In Australia, ambrosia beetles are not currently considered a significant pest in commercial Pinus plantations, where the bark beetle Ips grandicollis is known as the primary invader of stressed trees. However, in 2019, ambrosia beetles Xyleborus perforans and X. bispinatus, co-occurring with I. grandicollis, were found to have colonised a large proportion of drought-stressed trees in commercial Pinus plantations in north-east New South Wales. In this study, X. perforans (the most prevalent of two Xyleborus spp.) was collected from infested dead and dying trees in two NSW Pinus plantations. Fungal isolates of suspected Pinus pathogens were recovered from beetle mycangia and exoskeletons as well as ambrosia beetle galleries. Morphological examination and multilocus sequence analysis identified five fungi associated with X. perforans: Fusarium parceramosum, Fusarium aff. solani, Ophiostoma ips, Raffaelea deltoideospora and Sporothrix pseudoabietina. For Australia, this is the first report of F. parceramosum, as well as the first records of O. ips, R. deltoideospora and S. pseudoabietina being vectored by Xyleborus. Pathogenicity tests were performed on seedlings of three Pinus spp., with O. ips producing significantly longer lesions than the other fungi. This study demonstrates the potential for seemingly harmless ambrosia beetles to vector plant pathogens in Australian forests, providing a mode of disease transmission that should be considered in plantation management and forest biosecurity.

Funding

Peter Gillespie, Ainsley Seago and Manon Griffith for beetle identifications. Forestry Corporation of NSW for access to pine plantations.

History

Publication Date

2024-01-01

Journal

Australasian Plant Pathology

Volume

53

Issue

1

Pagination

12p. (p. 51-62)

Publisher

Springer Nature

ISSN

0815-3191

Rights Statement

© The Author(s) 2023. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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