Extracellular vesicles secreted by Saccharomyces cerevisiae are involved in cell wall remodelling

Zhao, Kening; Bleackley, Mark; Chisanga, David; Gangoda, Lahiru; Fonseka, Pamali; Liem, Michael; Kalra, Hina; Al Saffar, Haidar; Keerthikumar, Shivakumar; Ang, Ching-Seng; Adda, Christopher; Jiang, Lanzhou; Yap, Kuok; Poon, Ivan; Lock, Peter; Bulone, Vincent; Anderson, Marilyn; Mathivanan, Suresh

doi:10.1038/s42003-019-0538-8

Extracellular vesicles secreted by Saccharomyces cerevisiae are involved in cell wall remodelling

journal contribution

posted on 2025-01-07, 04:57 authored by Kening ZhaoKening Zhao, Mark BleackleyMark Bleackley, David Chisanga, Lahiru GangodaLahiru Gangoda, Pamali FonsekaPamali Fonseka, Michael LiemMichael Liem, Hina Kalra, Haidar Al Saffar, Shivakumar KeerthikumarShivakumar Keerthikumar, Ching-Seng Ang, Christopher AddaChristopher Adda, Lanzhou JiangLanzhou Jiang, Kuok Yap, Ivan PoonIvan Poon, Peter Lock, Vincent Bulone, Marilyn AndersonMarilyn Anderson, Suresh MathivananSuresh Mathivanan

Extracellular vesicles (EVs) are membranous vesicles that are released by cells. In this study, the role of the Endosomal Sorting Complex Required for Transport (ESCRT) machinery in the biogenesis of yeast EVs was examined. Knockout of components of the ESCRT machinery altered the morphology and size of EVs as well as decreased the abundance of EVs. In contrast, strains with deletions in cell wall biosynthesis genes, produced more EVs than wildtype. Proteomic analysis highlighted the depletion of ESCRT components and enrichment of cell wall remodelling enzymes, glucan synthase subunit Fks1 and chitin synthase Chs3, in yeast EVs. Interestingly, EVs containing Fks1 and Chs3 rescued the yeast cells from antifungal molecules. However, EVs from fks1∆ or chs3∆ or the vps23∆chs3∆ double knockout strain were unable to rescue the yeast cells as compared to vps23∆ EVs. Overall, we have identified a potential role for yeast EVs in cell wall remodelling.

Funding

Suresh Mathivanan is supported by Australian Research Council DECRA (DE150101777), Australian Research Council Discovery Project (DP170102312) and Australian Research Council Future Fellowship (FT180100333). Australian Research Council Discovery Project (DP160100309) supports the work of M.B., M.A. and V.B.

History

Publication Date

2019-08-09

Journal

Communications Biology

Volume

2

Article Number

305

Pagination

13p.

Publisher

Springer Nature

ISSN

2399-3642

Rights Statement

© The Author(s) 2019 This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.