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Extracellular vesicle isolation and characterization: toward clinical application
journal contributionposted on 2021-03-12, 06:57 authored by Rong Xu, David GreeningDavid Greening, H-J Zhu, N Takahashi, Richard SimpsonRichard Simpson
Two broad categories of extracellular vesicles (EVs), exosomes and shed microvesicles (sMVs), which differ in size distribution as well as protein and RNA profiles, have been described. EVs are known to play key roles in cell-cell communication, acting proximally as well as systemically. This Review discusses the nature of EV subtypes, strategies for isolating EVs from both cell-culture media and body fluids, and procedures for quantifying EVs. We also discuss proteins selectively enriched in exosomes and sMVs that have the potential for use as markers to discriminate between EV subtypes, as well as various applications of EVs in clinical diagnosis.
The authors were supported, in part, by the National Health and Medical Research Council (NHMRC) of Australia: project grants no. 1057741 (to R.J. Simpson and D.W. Greening) and no. 433619 (to H.J. Zhu) and a La Trobe University Leadership RFA grant (to D.W. Greening). This work was also supported by Operational Infrastructure Support Program funding provided by the Victorian Government. R. Xu is supported by a La Trobe University Post Graduate Fellowship. The authors acknowledge the input of Donna Dorow for proofreading this Review.
JournalJournal of Clinical Investigation
Pagination11p. (p. 1152-1162)
PublisherAmerican Society for Clinical Investigation
Rights StatementThe Author reserves all moral rights over the deposited text and must be credited if any re-use occurs. Documents deposited in OPAL are the Open Access versions of outputs published elsewhere. Changes resulting from the publishing process may therefore not be reflected in this document. The final published version may be obtained via the publisher’s DOI. Please note that additional copyright and access restrictions may apply to the published version.
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Science & TechnologyLife Sciences & BiomedicineMedicine, Research & ExperimentalResearch & Experimental MedicineNANOPARTICLE TRACKING ANALYSISCELL-DERIVED EXOSOMESDENSITY-GRADIENT SEPARATIONMULTICOLOR FLOW-CYTOMETRYMEMBRANE-VESICLESCANCER EXOSOMESMULTIVESICULAR BODIESPURIFIED EXOSOMESPLASMA-MEMBRANELARGE ONCOSOMESBody FluidsAnimalsHumansCulture MediaCell-Derived MicroparticlesExosomesBiomarkersImmunology