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Establishing a multiplex imaging panel to study T cell development in the thymus in mouse

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posted on 2022-10-21, 03:22 authored by Amr AllamAmr Allam, SM Russell
Multiplexed immunohistochemistry enables analysis of cellular and signaling events in the context of an intact organ. Here, we describe protocols for applying multiplexed immunohistochemistry to the mouse thymus. In particular, we describe how to identify cells at the specific differentiation stage known as β-selection, and to monitor pre-TCR signaling and the cellular response at that stage. For complete details on the use and execution of this protocol, please refer to Allam et al. (2021).

History

Publication Date

2022-09-16

Journal

STAR Protocols

Volume

3

Issue

3

Article Number

101472

Pagination

14p.

Publisher

Elsevier

ISSN

2666-1667

Rights Statement

© 2022 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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