La Trobe
1200896_Berkowitz,O_2022.pdf (19.83 MB)

Enhanced reactive oxygen detoxification occurs in salt-stressed soybean roots expressing GmSALT3

Download (19.83 MB)
journal contribution
posted on 2022-09-29, 02:29 authored by Y Qu, R Guan, L Yu, Oliver BerkowitzOliver Berkowitz, R David, James WhelanJames Whelan, M Ford, S Wege, L Qiu, M Gilliham
Soybean (Glycine max) is an important crop globally for food and edible oil production. Soybean plants are sensitive to salinity (NaCl), with significant yield decreases reported under saline conditions. GmSALT3 is the dominant gene underlying a major QTL for salt tolerance in soybean. GmSALT3 encodes a transmembrane protein belonging to the plant cation/proton exchanger (CHX) family, and is predominately expressed in root phloem and xylem associated cells under both saline and non-saline conditions. It is currently unknown through which molecular mechanism(s) the ER-localised GmSALT3 contributes to salinity tolerance, as its localisation excludes direct involvement in ion exclusion. In order to gain insights into potential molecular mechanism(s), we used RNA-seq analysis of roots from two soybean NILs (near isogenic lines); NIL-S (salt-sensitive, Gmsalt3), and NIL-T (salt-tolerant, GmSALT3), grown under control and saline conditions (200 mM NaCl) at three time points (0 h, 6 h, and 3 days). Gene ontology (GO) analysis showed that NIL-T has greater responses aligned to oxidation reduction. ROS were less abundant and scavenging enzyme activity was greater in NIL-T, consistent with the RNA-seq data. Further analysis indicated that genes related to calcium signalling, vesicle trafficking and Casparian strip (CS) development were upregulated in NIL-T following salt treatment. We propose that GmSALT3 improves the ability of NIL-T to cope with saline stress through preventing ROS overaccumulation in roots, and potentially modulating Ca2+ signalling, vesicle trafficking and formation of diffusion barriers.

Funding

Centre of Excellence in Plant Energy Biology, Australian Research Council, Grant/Award Number: CE140100008; Australian Research Council, Grant/Award Numbers: DE160100804, FT130100709; Chinese Academy of Agricultural Sciences; Natural Science Foundation of China, Grant/Award Number: 31830066

History

Publication Date

2022-05-01

Journal

Physiologia Plantarum

Volume

174

Issue

3

Article Number

e13709

Pagination

16p.

Publisher

John Wiley & Sons Ltd on behalf of Scandinavian Plant Physiology Society.

ISSN

0031-9317

Rights Statement

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. © 2022 The Authors.

Usage metrics

    Journal Articles

    Categories

    No categories selected

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC