Electroactive biofilms on surface functionalized anodes: the anode respiring behavior of a novel electroactive bacterium, Desulfuromonas acetexigens
journal contributionposted on 28.01.2021, 03:03 by KP Katuri, S Kamireddy, P Kavanagh, A Muhammad, Peter O Conghaile, A Kumar, PE Saikaly, D Leech
© 2020 Surface chemistry is known to influence the formation, composition, and electroactivity of electron-conducting biofilms. However, understanding of the evolution of microbial composition during biofilm development and its impact on the electrochemical response is limited. Here we present voltammetric, microscopic and microbial community analysis of biofilms formed under fixed applied potential for modified graphite electrodes during early (90 h) and mature (340 h) growth phases. Electrodes modified to introduce hydrophilic groups (-NH2, -COOH and -OH) enhance early-stage biofilm formation compared to unmodified or electrodes modified with hydrophobic groups (-C2H5). In addition, early-stage films formed on hydrophilic electrodes are dominated by the gram-negative sulfur-reducing bacterium Desulfuromonas acetexigens while Geobacter sp. dominates on -C2H5 and unmodified electrodes. As biofilms mature, current generation becomes similar, and D. acetexigens dominates in all biofilms irrespective of surface chemistry. Electrochemistry of pure culture D. acetexigens biofilms reveal that this microbe is capable of forming electroactive biofilms producing considerable current density of > 9 A/m2 in a short period of potential-induced growth (~19 h following inoculation) using acetate as an electron donor. The inability of D. acetexigens biofilms to use H2 as a sole source electron donor for current generation shows promise for maximizing H2 recovery in single-chambered microbial electrolysis cell systems treating wastewaters.
This work was supported by the Center Competitive Funding Program (Grant No. FCC/1/1971-05-01) and the Competitive Research Grant (URF/1/2985-01-01) from King Abdullah University of Science and Technology (KAUST) and by a Charles Parsons Energy Research Award 06/CPE006, through Science Foundation Ireland. Graphical abstract was developed using Biorender software.
Rights StatementThe Author reserves all moral rights over the deposited text and must be credited if any re-use occurs. Documents deposited in OPAL are the Open Access versions of outputs published elsewhere. Changes resulting from the publishing process may therefore not be reflected in this document. The final published version may be obtained via the publisher’s DOI. Please note that additional copyright and access restrictions may apply to the published version.
Science & TechnologyTechnologyLife Sciences & BiomedicinePhysical SciencesEngineering, EnvironmentalEnvironmental SciencesWater ResourcesEngineeringEnvironmental Sciences & EcologyMicrobial electrolysis cellElectroactive bacteriaFunctionalized anodeBiofilm anodeDesulfuromonas acetexigensHydrogenELECTROCHEMICAL MEMBRANE BIOREACTORMIXED CULTURE BIOFILMSGEOBACTER-SULFURREDUCENSELECTRICITY PRODUCTIONCHARGE-TRANSPORTCARBON ANODESCELLELECTRODEVOLTAMMETRYPERFORMANCEBiofilmsDesulfuromonasGeobacterElectrodesBioelectric Energy SourcesEnvironmental Engineering