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Effects of excess manganese on the xylem sap protein profile of tomato (Solanum lycopersicum) as revealed by shotgun proteomic analysis

journal contribution
posted on 04.01.2021, 01:32 by L Ceballos‐laita, E Gutierrez‐carbonell, D Takahashi, A Lonsdale, A Abadía, Monika DoblinMonika Doblin, Tony BacicTony Bacic, M Uemura, J Abadía, AF López‐millán
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Metal toxicity is a common problem in crop species worldwide. Some metals are naturally toxic, whereas others such as manganese (Mn) are essential micro‐nutrients for plant growth but can become toxic when in excess. Changes in the composition of the xylem sap, which is the main pathway for ion transport within the plant, is therefore vital to understanding the plant’s response(s) to metal toxicity. In this study we have assessed the effects of exposure of tomato roots to excess Mn on the protein profile of the xylem sap, using a shotgun proteomics approach. Plants were grown in nutrient solution using 4.6 and 300 μM MnCl2 as control and excess Mn treatments, respectively. This approach yielded 668 proteins reliably identified and quantified. Excess Mn caused statistically significant (at p ≤ 0.05) and biologically relevant changes in relative abundance (≥2‐fold increases or ≥50% decreases) in 322 proteins, with 82% of them predicted to be secretory using three different prediction tools, with more decreasing than increasing (181 and 82, respectively), suggesting that this metal stress causes an overall deactivation of metabolic pathways. Processes most affected by excess Mn were in the oxido‐reductase, polysaccharide and protein metabolism classes. Excess Mn induced changes in hydrolases and peroxidases involved in cell wall degradation and lignin formation, respectively, consistent with the existence of alterations in the cell wall. Protein turnover was also affected, as indicated by the decrease in proteolytic enzymes and protein synthesis‐related proteins. Excess Mn modified the redox environment of the xylem sap, with changes in the abundance of oxido‐reductase and defense protein classes indicating a stress scenario. Finally, results indicate that excess Mn decreased the amounts of proteins associated with several signaling pathways, including fasciclin‐like arabinogalactan‐proteins and lipids, as well as proteases, which may be involved in the release of signaling peptides and protein maturation. The comparison of the proteins changing in abundance in xylem sap and roots indicate the existence of tissue‐specific and systemic responses to excess Mn. Data are available via ProteomeXchange with identifier PXD021973.

Funding

Supported by the Spanish State Research Agency (AEI) co-financed by the European Regional Development Fund (FEDER) (project AGL2016-75226-R; AEI/FEDER, UE) and the Aragon Government (group A09-20R). Research conducted in Iwate University was in part supported by JSPS KAKENHI Grant Numbers 24-7373, 22120003, and 24370018. E.G.-C. was supported by a JAE Pre-CSIC contract and L.C.-L was supported by a FPI-MINECO contract.

History

Publication Date

23/11/2020

Journal

International Journal of Molecular Sciences

Volume

21

Issue

22

Article Number

8863

Pagination

28p. (p. 1-28)

Publisher

MDPI

ISSN

1422-0067

Rights Statement

The Author reserves all moral rights over the deposited text and must be credited if any re-use occurs. Documents deposited in OPAL are the Open Access versions of outputs published elsewhere. Changes resulting from the publishing process may therefore not be reflected in this document. The final published version may be obtained via the publisher’s DOI. Please note that additional copyright and access restrictions may apply to the published version.