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Diffraction data from aerosolized Coliphage PR772 virus particles imaged with the Linac Coherent Light Source

journal contribution
posted on 04.12.2020, 00:17 by Haoyuan Li, R Nazari, Brian AbbeyBrian Abbey, R Alvarez, A Aquila, K Ayyer, A Barty, Peter BerntsenPeter Berntsen, J Bielecki, A Pietrini, M Bucher, G Carini, HN Chapman, A Contreras, BJ Daurer, H DeMirci, L Flűckiger, M Frank, J Hajdu, MF Hantke, BG Hogue, A Hosseinizadeh, MS Hunter, HO Jönsson, RA Kirian, RP Kurta, D Loh, FRNC Maia, Adrian MancusoAdrian Mancuso, Andrew J Morgan, M McFadden, K Muehlig, A Munke, HKN Reddy, C Nettelblad, A Ourmazd, M Rose, P Schwander, M Marvin Seibert, JA Sellberg, RG Sierra, Z Sun, M Svenda, IA Vartanyants, P Walter, D Westphal, G Williams, PL Xavier, CH Yoon, S Zaare
© 2020, This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply. Single Particle Imaging (SPI) with intense coherent X-ray pulses from X-ray free-electron lasers (XFELs) has the potential to produce molecular structures without the need for crystallization or freezing. Here we present a dataset of 285,944 diffraction patterns from aerosolized Coliphage PR772 virus particles injected into the femtosecond X-ray pulses of the Linac Coherent Light Source (LCLS). Additional exposures with background information are also deposited. The diffraction data were collected at the Atomic, Molecular and Optical Science Instrument (AMO) of the LCLS in 4 experimental beam times during a period of four years. The photon energy was either 1.2 or 1.7 keV and the pulse energy was between 2 and 4 mJ in a focal spot of about 1.3 μm x 1.7 μm full width at half maximum (FWHM). The X-ray laser pulses captured the particles in random orientations. The data offer insight into aerosolised virus particles in the gas phase, contain information relevant to improving experimental parameters, and provide a basis for developing algorithms for image analysis and reconstruction.

History

Publication Date

19/11/2020

Journal

Scientific Data

Volume

7

Issue

1

Article Number

404

Pagination

12p.

Publisher

Nature Publishing Group

ISSN

2052-4463

Rights Statement

The Author reserves all moral rights over the deposited text and must be credited if any re-use occurs. Documents deposited in OPAL are the Open Access versions of outputs published elsewhere. Changes resulting from the publishing process may therefore not be reflected in this document. The final published version may be obtained via the publisher’s DOI. Please note that additional copyright and access restrictions may apply to the published version.

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