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Detection of Urinary Albumin Using a “Turn-on” Fluorescent Probe with Aggregation-Induced Emission Characteristics

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posted on 08.09.2021, 08:19 authored by Q Hu, Bicheng YaoBicheng Yao, TC Owyong, S Prashanth, C Wang, X Zhang, WWH Wong, Y Tang, Yuning HongYuning Hong
Human serum albumin (HSA) is a broadly used biomarker for the diagnosis of various diseases such as chronic kidney disease. Here, a fluorescent probe TC426 with aggregation-induced emission (AIE) characteristics is reported as a sensitive and specific probe for HSA. This probe is non-emissive in aqueous solution, meanwhile it shows bright fluorescence upon interacting with HSA, which makes it applicable in detecting HSA with a high signal to noise ratio. Besides, the fluorescence of TC426 exhibits a high linear correlation with the concentration of albumin in the range of microalbumin (20–200 mg/L), which has a significant importance for the early diagnosis of glomerulus related diseases. Compared with previously reported HSA probes TPE-4TA and BSPOTPE, TC426 shows comparable anti-interference ability towards creatinine and other major components in urine but is excited by a longer excitation wavelength at the visible light range. Finally, with the established assay, TC426 shows excellent performance in detecting HSA in real human urine, indicating its great potential in practical urinalysis.


We acknowledge the Australia-China Science and Research Fund, Joint Research Centre on Personal Health Technologies for support. T.C.O. and W.W.H.W. are supported by the ARC Centre of Excellence in Exciton Science (CE170100026).


Publication Date



Chemistry - An Asian Journal






8p. (p.1245-1252)





Rights Statement

This is the peer reviewed version of: Hu Q, Yao B, Owyong TC, Prashanth S, Wang C, Zhang X, Wong WWH, Tang Y, Hong Y. Detection of Urinary Albumin Using a "Turn-on" Fluorescent Probe with Aggregation-Induced Emission Characteristics. Chem Asian J. 2021 May 17;16(10):1245-1252. doi: 10.1002/asia.202100180. Epub 2021 Apr 1. PMID: 33759376, published in final form at This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions; it may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. Any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.