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Decrease in Plasma miR-27a and miR-221 After Concussion in Australian Football Players

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posted on 2022-03-30, 03:39 authored by SR Shultz, Caroline TaylorCaroline Taylor, R Aggio-Bruce, WT O’Brien, M Sun, AV Cioanca, G Neocleous, GF Symons, RD Brady, AA Hardikar, MV Joglekar, DM Costello, TJ O’Brien, R Natoli, Stuart McDonaldStuart McDonald
Introduction: Sports-related concussion (SRC) is a common form of brain injury that lacks reliable methods to guide clinical decisions. MicroRNAs (miRNAs) can influence biological processes involved in SRC, and measurement of miRNAs in biological fluids may provide objective diagnostic and return to play/recovery biomarkers. Therefore, this prospective study investigated the temporal profile of circulating miRNA levels in concussed male and female athletes. Methods: Pre-season baseline blood samples were collected from amateur Australian rules football players (82 males, 45 females). Of these, 20 males and 8 females sustained an SRC during the subsequent season and underwent blood sampling at 2-, 6- and 13-days post-injury. A miRNA discovery Open Array was conducted on plasma to assess the expression of 754 known/validated miRNAs. miRNA target identified were further investigated with quantitative real-time PCR (qRT-PCR) in a validation study. Data pertaining to SRC symptoms, demographics, sporting history, education history and concussion history were also collected. Results: Discovery analysis identified 18 candidate miRNA. The consequent validation study found that plasma miR-221-3p levels were decreased at 6d and 13d, and that miR-27a-3p levels were decreased at 6d, when compared to baseline. Moreover, miR-27a and miR-221-3p levels were inversely correlated with SRC symptom severity. Conclusion: Circulating levels of miR-27a-3p and miR-221-3p were decreased in the sub-acute stages after SRC, and were inversely correlated with SRC symptom severity. Although further studies are required, these analyses have identified miRNA biomarker candidates of SRC severity and recovery that may one day assist in its clinical management.


The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: A.A.H. and M.V.J. were supported through JDRF Australia CDA and a JDRF International Advanced-post-doctoral awards respectively.


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Biomarker Insights



Article Number

ARTN 11772719221081318







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© The Author(s) 2022. Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License ( which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (