La Trobe

Current and Future Advances in the Detection and Surveillance of Biosecurity-Relevant Equine Bacterial Diseases Using Loop-Mediated Isothermal Amplification (LAMP)

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posted on 2023-11-06, 06:48 authored by Alexandra KnoxAlexandra Knox, Gemma ZernaGemma Zerna, Travis BeddoeTravis Beddoe
Horses play an important role throughout the world, whether for work, culture, or leisure, providing an ever-growing significant contribution to the economy. The increase in importation and movement of horses, both nationally and internationally, has inevitably allowed for the global equine industry to grow. Subsequently, however, the potential for transmission of fatal equine bacterial diseases has also escalated, and devasting outbreaks continue to occur. To prevent such events, disease surveillance and diagnosis must be heightened throughout the industry. Current common, or “gold-standard” techniques, have shown to be inadequate at times, thus requiring newer technology to impede outbreaks. Loop-mediated isothermal amplification (LAMP) has proven to be a reliable, rapid, and accessible tool in both diagnostics and surveillance. This review will discuss equine bacterial diseases of biosecurity relevance and their current diagnostic approaches, as well as their respective LAMP assay developments. Additionally, we will provide insight regarding newer technology and advancements associated with this technique and their potential use for the outlined diseases.

Funding

This research was supported by the Cooperative Research Centres Project (CRC-P) awarded to Geneworks and La Trobe University. A.K. is supported by a La Trobe Industry Ph.D. scholarship and the Defence Science Institute, an initiative of the State Government of Victoria.

History

Publication Date

2023-08-18

Journal

Animals

Volume

13

Issue

16

Article Number

2663

Pagination

29p.

Publisher

Multidisciplinary Digital Publishing Institute

ISSN

2076-2615

Rights Statement

© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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