La Trobe
fpls-12-640919.pdf (3.03 MB)

Cracking the “Sugar Code”: A Snapshot of N- and O-Glycosylation Pathways and Functions in Plants Cells

Download (3.03 MB)
journal contribution
posted on 12.04.2021, 04:56 by R Strasser, G Seifert, Monika Doblin, Kim Johnson, C Ruprecht, F Pfrengle, Tony Bacic, JM Estevez
Glycosylation is a fundamental co-translational and/or post-translational modification process where an attachment of sugars onto either proteins or lipids can alter their biological function, subcellular location and modulate the development and physiology of an organism. Glycosylation is not a template driven process and as such produces a vastly larger array of glycan structures through combinatorial use of enzymes and of repeated common scaffolds and as a consequence it provides a huge expansion of both the proteome and lipidome. While the essential role of N- and O-glycan modifications on mammalian glycoproteins is already well documented, we are just starting to decode their biological functions in plants. Although significant advances have been made in plant glycobiology in the last decades, there are still key challenges impeding progress in the field and, as such, holistic modern high throughput approaches may help to address these conceptual gaps. In this snapshot, we present an update of the most common O- and N-glycan structures present on plant glycoproteins as well as (1) the plant glycosyltransferases (GTs) and glycosyl hydrolases (GHs) responsible for their biosynthesis; (2) a summary of microorganism-derived GHs characterized to cleave specific glycosidic linkages; (3) a summary of the available tools ranging from monoclonal antibodies (mAbs), lectins to chemical probes for the detection of specific sugar moieties within these complex macromolecules; (4) selected examples of N- and O-glycoproteins as well as in their related GTs to illustrate the complexity on their mode of action in plant cell growth and stress responses processes, and finally (5) we present the carbohydrate microarray approach that could revolutionize the way in which unknown plant GTs and GHs are identified and their specificities characterized.

Funding

This work was supported by grants from the Austrian Science Fund (FWF) P31920-B22 to RS and P32332-B to GS, the German Research Council (DFG) PF850/1-1 and PF850/7-1 to FP, the Australia Research Council to the ARC Centre of Excellence in Plant Cell Walls (CE1101007) to AB, MD, and KJ, and ANPCyT (PICT2016-0132 and PICT2017-0066), Fondo Nacional de Desarrollo Cientifico y Tecnologico (1200010), and Instituto Milenio iBio - Iniciativa Cientifica Milenio, MINECON to JE.

History

Publication Date

19/02/2021

Journal

Frontiers in Plant Science

Volume

12

Article Number

ARTN 640919

Pagination

19p.

Publisher

FRONTIERS MEDIA SA

ISSN

1664-462X

Rights Statement

The Author reserves all moral rights over the deposited text and must be credited if any re-use occurs. Documents deposited in OPAL are the Open Access versions of outputs published elsewhere. Changes resulting from the publishing process may therefore not be reflected in this document. The final published version may be obtained via the publisher’s DOI. Please note that additional copyright and access restrictions may apply to the published version.

Licence

Exports