Coordination between zinc and phosphate homeostasis involves the transcription factor PHR1, the phosphate exporter PHO1, and its homologue PHO1;H3 in Arabidopsis
journal contributionposted on 12.04.2021, 00:10 by GA Khan, S Bouraine, S Wege, Y Li, MD Carbonnel, P Berthomieu, Y Poirier, H Rouached
Interactions between zinc (Zn) and phosphate (Pi) nutrition in plants have long been recognized, but little information is available on their molecular bases and biological significance. This work aimed at examining the effects of Zn deficiency on Pi accumulation in Arabidopsis thaliana and uncovering genes involved in the Zn-Pi synergy. Wild-type plants as well as mutants affected in Pi signalling and transport genes, namely the transcription factor PHR1, the E2-conjugase PHO2, and the Pi exporter PHO1, were examined. Zn deficiency caused an increase in shoot Pi content in the wild type as well as in the pho2 mutant, but not in the phr1 or pho1 mutants. This indicated that PHR1 and PHO1 participate in the coregulation of Zn and Pi homeostasis. Zn deprivation had a very limited effect on transcript levels of Pi-starvation-responsive genes such as AT4, IPS1, and microRNA399, or on of members of the high-affinity Pi transporter family PHT1. Interestingly, one of the PHO1 homologues, PHO1;H3, was upregulated in response to Zn deficiency. The expression pattern of PHO1 and PHO1;H3 were similar, both being expressed in cells of the root vascular cylinder and both localized to the Golgi when expressed transiently in tobacco cells. When grown in Zn-free medium, pho1;h3 mutant plants displayed higher Pi contents in the shoots than wild-type plants. This was, however, not observed in a pho1 pho1;h3 double mutant, suggesting that PHO1;H3 restricts root-to-shoot Pi transfer requiring PHO1 function for Pi homeostasis in response to Zn deficiency. © 2014 The Author.
The authors are grateful to Javier Paz-Ares (CSIC, Madrid) for providing seeds of the phr1 mutant. Authors wish to thank Laurent Nussaume for helpful discussions. This work was funded by the Swiss National Foundation (31003A-12293 and 31003A-138339 to YP), a Marie-Curie Intra-European-Fellowship (298843 'PHOSTASIA' to SW), and the Institut National de la Recherche Agronomique (to HR). The authors acknowledge the contribution of the Cellular Imaging Facility of the University of Lausanne, Switzerland.
JournalJournal of Experimental Botany
PublisherOxford University Press
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Science & TechnologyLife Sciences & BiomedicinePlant SciencesHomeostasisinteractionphosphatesignallingtransportzincMETAL TRANSPORTERSSTARVATION RESPONSEINORGANIC-PHOSPHATESHORT HYPOCOTYLK+ NUTRITIONCROSS-TALKIN-VIVODEFICIENCYEXPRESSIONPLANTSzinc.ArabidopsisArabidopsis ProteinsBiological TransportGene Expression Regulation, PlantGenes, ReporterGolgi ApparatusPhosphatesPlant RootsPlant ShootsPlants, Genetically ModifiedRNA, MessengerRNA, PlantRecombinant Fusion ProteinsSignal TransductionTobaccoTranscription FactorsZincPlant Biology & Botany