Convergent evolution of defensin sequence, structure and function
journal contributionposted on 2021-01-05, 22:30 authored by Thomas ShafeeThomas Shafee, Fung LayFung Lay, Thanh PhanThanh Phan, Marilyn AndersonMarilyn Anderson, Mark HulettMark Hulett
© 2016, Springer International Publishing. Defensins are a well-characterised group of small, disulphide-rich, cationic peptides that are produced by essentially all eukaryotes and are highly diverse in their sequences and structures. Most display broad range antimicrobial activity at low micromolar concentrations, whereas others have other diverse roles, including cell signalling (e.g. immune cell recruitment, self/non-self-recognition), ion channel perturbation, toxic functions, and enzyme inhibition. The defensins consist of two superfamilies, each derived from an independent evolutionary origin, which have subsequently undergone extensive divergent evolution in their sequence, structure and function. Referred to as the cis- and trans-defensin superfamilies, they are classified based on their secondary structure orientation, cysteine motifs and disulphide bond connectivities, tertiary structure similarities and precursor gene sequence. The utility of displaying loops on a stable, compact, disulphide-rich core has been exploited by evolution on multiple occasions. The defensin superfamilies represent a case where the ensuing convergent evolution of sequence, structure and function has been particularly extreme. Here, we discuss the extent, causes and significance of these convergent features, drawing examples from across the eukaryotes.
The authors would like to thank the financial support of the Australian Research council (Grant DP150104386), Hexima Ltd, and La Trobe University.
Australian Research Council | DP150104386
La Trobe University
JournalCellular and Molecular Life Sciences
Pagination20p. (p. 663-682)
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Science & TechnologyLife Sciences & BiomedicineBiochemistry & Molecular BiologyCell BiologyDisulphide-rich proteinProtein superfamilyEvolutionary constraintDivergent evolutionEvolvabilityAntimicrobial peptideANTIMICROBIAL PEPTIDE DATABASEANTIFUNGAL PLANT DEFENSINANTIGEN-PRESENTING CELLSALPHA-AMYLASE INHIBITORSCOPY NUMBERFUNGAL DEFENSINDENDRITIC CELLSTHETA-DEFENSINSANTIBACTERIAL PEPTIDEHUMAN BETA-DEFENSIN-3AnimalsHumansDefensinsAnti-Infective AgentsSequence AlignmentEvolution, MolecularPhylogenyAmino Acid SequenceProtein ConformationGene DosageModels, Molecular