Coherent diffraction of single Rice Dwarf virus particles using hard X-rays at the Linac Coherent Light Source
journal contributionposted on 2023-03-31, 03:44 authored by A Munke, J Andreasson, A Aquila, S Awel, K Ayyer, A Barty, RJ Bean, Peter Berntsen, J Bielecki, S Boutet, M Bucher, HN Chapman, BJ Daurer, H DeMirci, V Elser, P Fromme, J Hajdu, MF Hantke, A Higashiura, BG Hogue, A Hosseinizadeh, Y Kim, RA Kirian, HKN Reddy, T-Y Lan, DSD Larsson, H Liu, ND Loh, FRNC Maia, Adrian MancusoAdrian Mancuso, K Muhlig, A Nakagawa, D Nam, G Nelson, C Nettelblad, K Okamoto, A Ourmazd, M Rose, G van der Schot, P Schwander, MM Seibert, JA Sellberg, RG Sierra, C Song, M Svenda, N Timneanu, IA Vartanyants, D Westphal, MO Wiedorn, GJ Williams, PL Xavier, CH Yoon, J Zook
Single particle diffractive imaging data from Rice Dwarf Virus (RDV) were recorded using the Coherent X-ray Imaging (CXI) instrument at the Linac Coherent Light Source (LCLS). RDV was chosen as it is a well-characterized model system, useful for proof-of-principle experiments, system optimization and algorithm development. RDV, an icosahedral virus of about 70 nm in diameter, was aerosolized and injected into the approximately 0.1 μm diameter focused hard X-ray beam at the CXI instrument of LCLS. Diffraction patterns from RDV with signal to 5.9 Ångström were recorded. The diffraction data are available through the Coherent X-ray Imaging Data Bank (CXIDB) as a resource for algorithm development, the contents of which are described here.