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Closed head experimental traumatic brain injury increases size and bone volume of callus in mice with concomitant tibial fracture

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posted on 2023-03-10, 04:59 authored by Rhys D Brady, Brian GrillsBrian Grills, Jarrod ChurchJarrod Church, NC Walsh, Aaron McDonaldAaron McDonald, DV Agoston, M Sun, TJ O'Brien, SR Shultz, Stuart McDonaldStuart McDonald
Concomitant traumatic brain injury (TBI) and long bone fracture are commonly observed in multitrauma and polytrauma. Despite clinical observations of enhanced bone healing in patients with TBI, the relationship between TBI and fracture healing remains poorly understood, with clinical data limited by the presence of several confounding variables. Here we developed a novel trauma model featuring closed-skull weight-drop TBI and concomitant tibial fracture in order to investigate the effect of TBI on fracture healing. Male mice were assigned into Fracture + Sham TBI (FX) or Fracture + TBI (MULTI) groups and sacrificed at 21 and 35 days post-injury for analysis of healing fractures by micro computed tomography (μCT) and histomorphometry. μCT analysis revealed calluses from MULTI mice had a greater bone and total tissue volume, and displayed higher mean polar moment of inertia when compared to calluses from FX mice at 21 days post-injury. Histomorphometric results demonstrated an increased amount of trabecular bone in MULTI calluses at 21 days post-injury. These findings indicate that closed head TBI results in calluses that are larger in size and have an increased bone volume, which is consistent with the notion that TBI induces the formation of a more robust callus.

Funding

This work was supported by grants to SS and TO from the NHMRC, a fellowship to SS from CIHR, funding from La Trobe University Understanding Disease RFA to SM and La Trobe University School of Life Science Postgraduate Publication Award to RB.

History

Publication Date

2016-09-29

Journal

Scientific Reports

Volume

6

Article Number

34491

Pagination

9p.

Publisher

Nature Publishing Group

ISSN

2045-2322

Rights Statement

© The Author(s) 2016 This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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