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Cell wall modification by the xyloglucan endotransglucosylase/hydrolase XTH19 influences freezing tolerance after cold and sub-zero acclimation

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posted on 21.04.2021, 04:54 by D Takahashi, Kim Johnson, Pengfei Hao, T Tuong, A Erban, A Sampathkumar, Tony Bacic, DP Livingston, J Kopka, T Kuroha, R Yokoyama, K Nishitani, E Zuther, DK Hincha
Freezing triggers extracellular ice formation leading to cell dehydration and deformation during a freeze–thaw cycle. Many plant species increase their freezing tolerance during exposure to low, non-freezing temperatures, a process termed cold acclimation. In addition, exposure to mild freezing temperatures after cold acclimation evokes a further increase in freezing tolerance (sub-zero acclimation). Previous transcriptome and proteome analyses indicate that cell wall remodelling may be particularly important for sub-zero acclimation. In the present study, we used a combination of immunohistochemical, chemical and spectroscopic analyses to characterize the cell walls of Arabidopsis thaliana and characterized a mutant in the XTH19 gene, encoding a xyloglucan endotransglucosylase/hydrolase (XTH). The mutant showed reduced freezing tolerance after both cold and sub-zero acclimation, compared to the Col-0 wild type, which was associated with differences in cell wall composition and structure. Most strikingly, immunohistochemistry in combination with 3D reconstruction of centres of rosette indicated that epitopes of the xyloglucan-specific antibody LM25 were highly abundant in the vasculature of Col-0 plants after sub-zero acclimation but absent in the XTH19 mutant. Taken together, our data shed new light on the potential roles of cell wall remodelling for the increased freezing tolerance observed after low temperature acclimation.

Funding

Alexander von Humboldt-Stiftung, Grant/Award Number: Postdoctoral Fellowship; Deutsche Forschungsgemeinschaft, Grant/Award Number: CRC 973, A3; Japan Society for the Promotion of Science, Grant/Award Number: 27328, 20K15494

History

Publication Date

01/03/2021

Journal

Plant Cell and Environment

Volume

44

Issue

3

Pagination

16p. (p. 915-930)

Publisher

Wiley

ISSN

0140-7791

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The Author reserves all moral rights over the deposited text and must be credited if any re-use occurs. Documents deposited in OPAL are the Open Access versions of outputs published elsewhere. Changes resulting from the publishing process may therefore not be reflected in this document. The final published version may be obtained via the publisher’s DOI. Please note that additional copyright and access restrictions may apply to the published version.

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