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Cell culture‐derived extracellular vesicles: Considerations for reporting cell culturing parameters

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posted on 2024-04-17, 00:26 authored by Faezeh Shekari, Faisal J Alibhai, Hossein Baharvand, Verena Börger, Stefania Bruno, Owen Davies, Bernd Giebel, Mario Gimona, Ghasem Hosseini Salekdeh, Lorena Martin‐Jaular, Suresh MathivananSuresh Mathivanan, Inge Nelissen, Esther Nolte‐’t Hoen, Lorraine O'Driscoll, Francesca Perut, Stefano Pluchino, Gabriella Pocsfalvi, Carlos Salomon, Carolina Soekmadji, Simon Staubach, Ana Claudia Torrecilhas, Ganesh Vilas Shelke, Tobias Tertel, Dandan Zhu, Clotilde Théry, Kenneth Witwer, Rienk Nieuwland

Cell culture‐conditioned medium (CCM) is a valuable source of extracellular vesicles (EVs) for basic scientific, therapeutic and diagnostic applications. Cell culturing parameters affect the biochemical composition, release and possibly the function of CCM‐derived EVs (CCM‐EV). The CCM‐EV task force of the Rigor and Standardization Subcommittee of the International Society for Extracellular Vesicles aims to identify relevant cell culturing parameters, describe their effects based on current knowledge, recommend reporting parameters and identify outstanding questions. While some recommendations are valid for all cell types, cell‐specific recommendations may need to be established for non‐mammalian sources, such as bacteria, yeast and plant cells. Current progress towards these goals is summarized in this perspective paper, along with a checklist to facilitate transparent reporting of cell culturing parameters to improve the reproducibility of CCM‐EV research.

Funding

International Society for Extracellular Vesicles (ISEV)

History

Publication Date

2023-10-01

Journal

Journal of Extracellular Biology

Volume

2

Issue

10

Article Number

e115

Pagination

25p.

Publisher

Wiley

ISSN

2768-2811

Rights Statement

© 2023 The Authors. Journal of Extracellular Biology published by Wiley Periodicals LLC on behalf of International Society for Extracellular Vesicles. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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