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Application of genomics to understand salt tolerance in lentil

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journal contribution
posted on 20.04.2021, 22:28 by R Dissanayake, Noel CoganNoel Cogan, KF Smith, S Kaur
Soil salinity is a major abiotic stress, limiting lentil productivity worldwide. Understanding the genetic basis of salt tolerance is vital to develop tolerant varieties. A diversity panel consist-ing of 276 lentil accessions was screened in a previous study through traditional and image‐based approaches to quantify growth under salt stress. Genotyping was performed using two contrasting methods, targeted (tGBS) and transcriptome (GBS‐t) genotyping‐by‐sequencing, to evaluate the most appropriate methodology. tGBS revealed the highest number of single‐base variants (SNPs) (c. 56,349), and markers were more evenly distributed across the genome compared to GBS‐t. A genome‐wide association study (GWAS) was conducted using a mixed linear model. Significant marker‐trait associations were observed on Chromosome 2 as well as Chromosome 4, and a range of candidate genes was identified from the reference genome, the most plausible being potassium transporters, which are known to be involved in salt tolerance in related species. Detailed mineral composition performed on salt‐treated and control plant tissues revealed the salt tolerance mechanism in lentil, in which tolerant accessions do not transport Na ions around the plant instead local-ize within the root tissues. The pedigree analysis identified two parental accessions that could have been the key sources of tolerance in this dataset. +

Funding

This work was supported by funding from Agriculture Victoria (Department of Jobs, Precincts and Regions, State Government of Victoria, Australia) and a Melbourne Research Scholarship from The University of Melbourne, Australia.

History

Publication Date

01/03/2021

Journal

Genes

Volume

12

Issue

3

Article Number

332

Pagination

p. 1-18

Publisher

MDPI

ISSN

2073-4425

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