Application of genomics to understand salt tolerance in lentil

Soil salinity is a major abiotic stress, limiting lentil productivity worldwide. Understanding the genetic basis of salt tolerance is vital to develop tolerant varieties. A diversity panel consist-ing of 276 lentil accessions was screened in a previous study through traditional and image‐based approaches to quantify growth under salt stress. Genotyping was performed using two contrasting methods, targeted (tGBS) and transcriptome (GBS‐t) genotyping‐by‐sequencing, to evaluate the most appropriate methodology. tGBS revealed the highest number of single‐base variants (SNPs) (c. 56,349), and markers were more evenly distributed across the genome compared to GBS‐t. A genome‐wide association study (GWAS) was conducted using a mixed linear model. Significant marker‐trait associations were observed on Chromosome 2 as well as Chromosome 4, and a range of candidate genes was identified from the reference genome, the most plausible being potassium transporters, which are known to be involved in salt tolerance in related species. Detailed mineral composition performed on salt‐treated and control plant tissues revealed the salt tolerance mechanism in lentil, in which tolerant accessions do not transport Na ions around the plant instead local-ize within the root tissues. The pedigree analysis identified two parental accessions that could have been the key sources of tolerance in this dataset. +