La Trobe

An intrasplenic injection model of pancreatic cancer metastasis to the liver in mice

journal contribution
posted on 2024-12-20, 02:03 authored by Megan O'BrienMegan O'Brien, Matthias ErnstMatthias Ernst, Ashleigh PohAshleigh Poh

Here, we provide a protocol for an intrasplenic injection model to establish pancreatic tumors in the mouse liver. We describe the steps to inject tumor cells into mouse spleen and to perform a splenectomy, followed by animal recovery and end point analysis of tumors in the liver. This model allows rapid and reproducible tumor growth in a clinically relevant metastatic site, providing a platform to evaluate the efficacy of anti-cancer drugs. This technique can be expanded to other cancer cell lines.

Funding

M.E. received funding from Ludwig Cancer Research and is an NHMRC Investigator grant recipient (1173814) . A.R.P. received support from 2018 Priority-driven Collaborative Cancer Research Scheme Grant (1157894) co -funded by Cancer Australia, Cure Cancer, and Pancare Foundation; a Tour de Cure Early Career Seed Grant (RSP-060-18/19) ; and an Avner Collaboration Grant from PanKind, The Australian Pancreatic Cancer Foundation in collaboration with Tour de Cure, and Woolworths Limited through Woolies on Wheels and Walks. A.R.P. is an NHMRC Peter Doherty Early Career Fellow (1166447) .

History

Publication Date

2023-03-17

Journal

STAR Protocols

Volume

4

Issue

1

Article Number

102021

Pagination

16p.

Publisher

Elsevier

ISSN

2666-1667

Rights Statement

© 2022 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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    Categories

    Keywords

    Cell BiologyCell cultureCancerImmunologyModel Organisms

    Licence

    CC BY-NC-ND 4.0

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