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A multi-million image Serial Femtosecond Crystallography dataset collected at the European XFEL

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posted on 2022-05-09, 07:03 authored by HJ Kirkwood, R de Wijn, G Mills, R Letrun, M Kloos, M Vakili, M Karnevskiy, K Ahmed, RJ Bean, J Bielecki, F Dall’Antonia, Y Kim, C Kim, J Koliyadu, A Round, T Sato, M Sikorski, P Vagovič, J Sztuk-Dambietz, Adrian MancusoAdrian Mancuso
Serial femtosecond crystallography is a rapidly developing method for determining the structure of biomolecules for samples which have proven challenging with conventional X-ray crystallography, such as for membrane proteins and microcrystals, or for time-resolved studies. The European XFEL, the first high repetition rate hard X-ray free electron laser, provides the ability to record diffraction data at more than an order of magnitude faster than previously achievable, putting increased demand on sample delivery and data processing. This work describes a publicly available serial femtosecond crystallography dataset collected at the SPB/SFX instrument at the European XFEL. This dataset contains information suitable for algorithmic development for detector calibration, image classification and structure determination, as well as testing and training for future users of the European XFEL and other XFELs.


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Springer Nature



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© The Author(s) 2022. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Cre-ative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not per-mitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit

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