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AAM_809304_OwYong,T_2020.pdf (1.21 MB)

A molecular chameleon for mapping subcellular polarity of unfolded proteome environment

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journal contribution
posted on 10.09.2021, 07:34 by Tze Cin Owyong, Pramod SubediPramod Subedi, Jieru DengJieru Deng, Elizabeth Hinde, Jason PaxmanJason Paxman, Jonathan M White, Weisan ChenWeisan Chen, Begona HerasBegona Heras, Wallace WH Wong, Yuning HongYuning Hong
Environmental polarity is an important factor that drives biomolecular interactions to regulate cell function. Herein, a general method of using the fluorogenic probe NTPAN-MI is reported to quantify the subcellular polarity change in response to protein unfolding. NTPAN-MI fluorescence is selectively activated upon labeling unfolded proteins with exposed thiols, thereby reporting on the extent of proteostasis. NTPAN-MI also reveals the collapse of the host proteome caused by influenza A virus infection. The emission profile of NTPAN-MI contains information of the local polarity of the unfolded proteome, which can be resolved through spectral phasor analysis. Under stress conditions that disrupt different checkpoints of protein quality control, distinct patterns of dielectric constant distribution in the cytoplasm can be observed. However, in the nucleus, the unfolded proteome was found to experience a more hydrophilic environment across all the stress conditions, indicating the central role of nucleus in the stress response process.

History

Publication Date

15/06/2020

Journal

Angewandte Chemie International Edition

Volume

59

Issue

25

Pagination

7p. (p. 10129-10135)

Publisher

Wiley

ISSN

1433-7851

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The Author reserves all moral rights over the deposited text and must be credited if any re-use occurs. Documents deposited in OPAL are the Open Access versions of outputs published elsewhere. Changes resulting from the publishing process may therefore not be reflected in this document. The final published version may be obtained via the publisher’s DOI. Please note that additional copyright and access restrictions may apply to the published version.

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