A Maleimide-functionalized Tetraphenylethene for Measuring and Imaging Unfolded Proteins in Cells

Zhang, Shouxiang; Liu, Mengjie; Tan, Lewis Yi Fong; Hong, Quentin; Pow, Ze Liang; Owyong, Tze Cin; Ding, Siyang; Wong, Wallace WH; Hong, Yuning

doi:10.1002/asia.201900150

A Maleimide-functionalized Tetraphenylethene for Measuring and Imaging Unfolded Proteins in Cells

journal contribution

posted on 2025-04-14, 01:05 authored by Shouxiang ZhangShouxiang Zhang, Mengjie Liu, Lewis Yi Fong Tan, Quentin Hong, Ze Liang Pow, Tze Cin OwyongTze Cin Owyong, Siyang DingSiyang Ding, Wallace WH Wong, Yuning HongYuning Hong

Collapse of the protein homeostasis (proteostasis) can lead to accumulation and aggregation of unfolded proteins, which has been found to associate with a number of disease conditions including neurodegenerative diseases, diabetes and inflammation. Here we report a maleimide-functionalized tetraphenylethene (TPE)-derivatized fluorescent dye, TPE-NMI, which shows fluorescence turn-on property upon reacting with unfolded proteins in vitro and in live cells under proteostatic stress conditions. The level of unfolded proteins can be measured by flow cytometry and visualized with confocal microscopy.<p></p>

Funding

This work was supported by grants to Y.H. (Australian Research Council DE170100058) and Rebecca L. Cooper (Medical Research Foundation PG2018043). WWHW acknowledges the Australian Research Council (ARC) Future Fellowship Scheme (FT130100500) and the ARC Centre of Excellence in Exciton Science (CE170100026) for supporting this work.

History

Publication Date

2019-01-01

Journal

Chemistry: An Asian Journal

Volume

14

Issue

6

Pagination

6p. (p. 904-909)

Publisher

Wiley

ISSN

1861-4728

Rights Statement

© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. This is the peer reviewed version of the following article: Zhang S, et al (2019). A Maleimide-functionalized Tetraphenylethene for Measuring and Imaging Unfolded Proteins in Cells. Chemistry - An Asian Journal, 14(6), 904-909, which has been published in final form at http://doi.org/10.1002/asia.201900150. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.