Antimicrobial Potential of Terpene Isolates of Cannabis sativa against Staphylococcus pseudintermedius: A Novel Approach to Combat Antimicrobial Resistance in Veterinary Medicine: raw data

Staphylococcus pseudintermedius is a harmful bacterium that affects dogs, causing various infections like canine pyoderma, otitis externa, and systemic issues in the urinary, respiratory, and reproductive systems. Canine pyoderma, an infection of a dog's skin and hair follicles, is a leading reason for antibiotic prescriptions in dogs. The excessive prescription of antibiotics has resulted in a rise in multidrug-resistant S. pseudintermedius and the emergence of methicillin-resistant S. pseudintermedius (MRSP) which pose significant challenges in treating infections, highlighting the need for alternative therapeutic strategies. Given the rising antimicrobial resistance (AMR) and limited antibiotic options, the potential of plant-derived terpenes as novel antimicrobial agents is being explored. Here we investigated the antimicrobial efficacy of eight terpenes commonly found in Cannabis sativa against clinical isolates of S. pseudintermedius. The study evaluates the antibacterial and bactericidal activities of these terpenes, their effects on bacterial ultrastructure, and time-kill kinetics. Among the terpenes tested, nerolidol emerged as particularly effective, demonstrating potent antibacterial activity and low cytotoxicity, with its efficacy influenced by its hydrophobicity and hydroxyl group positioning. Our findings contribute to understanding the antibacterial action of Cannabis terpenes, underscoring their potential as innovative solutions in veterinary medicine for combating drug-resistant pathogens such as S. pseudintermedius.