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Enrichment of human platelet membranes for proteomic analysis

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posted on 10.08.2021, 02:36 by David GreeningDavid Greening, Kristen M. Glenister, Rosemary L. Sparrow, Richard SimpsonRichard Simpson
This protocol details the extraction and purification of platelet membrane proteins from whole blood using SDS-PAGE in conjunction with LC-MS/MS. This method allowed the identification, and characterization of 207 platelet membrane proteins (PMP) from approximately 9.95 x 10(9) platelets (16).

History

Publication Date

01/01/2009

Book Title

Membrane Proteomics

Editors

Matthew Peirce Robin Wait

Publisher

Humana Press

Place of publication

New York, NY, USA

Series

Methods in Molecular Biology

Volume

528

Pagination

14p. (p.245-258)

ISBN-13

978-1-60327-310-7

Rights Statement

The Author reserves all moral rights over the deposited text and must be credited if any re-use occurs. Documents deposited in OPAL are the Open Access versions of outputs published elsewhere. Changes resulting from the publishing process may therefore not be reflected in this document. The final published version may be obtained via the publisher’s DOI. Please note that additional copyright and access restrictions may apply to the published version.

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