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Enrichment of human platelet membranes for proteomic analysis
chapterposted on 10.08.2021, 02:36 by David GreeningDavid Greening, Kristen M. Glenister, Rosemary L. Sparrow, Richard SimpsonRichard Simpson
This protocol details the extraction and purification of platelet membrane proteins from whole blood using SDS-PAGE in conjunction with LC-MS/MS. This method allowed the identification, and characterization of 207 platelet membrane proteins (PMP) from approximately 9.95 x 10(9) platelets (16).