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Enrichment of human platelet membranes for proteomic analysis
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posted on 2021-08-10, 02:36 authored by David GreeningDavid Greening, Kristen M. Glenister, Rosemary L. Sparrow, Richard SimpsonRichard SimpsonThis protocol details the extraction and purification of platelet membrane proteins from whole blood using SDS-PAGE in conjunction with LC-MS/MS. This method allowed the identification, and characterization of 207 platelet membrane proteins (PMP) from approximately 9.95 x 10(9) platelets (16).
History
Publication Date
2009-01-01Book Title
Membrane ProteomicsEditors
Matthew Peirce Robin WaitPublisher
Humana PressPlace of publication
New York, NY, USASeries
Methods in Molecular BiologyVolume
528Pagination
14p. (p.245-258)ISBN-13
978-1-60327-310-7Rights Statement
The Author reserves all moral rights over the deposited text and must be credited if any re-use occurs. Documents deposited in OPAL are the Open Access versions of outputs published elsewhere. Changes resulting from the publishing process may therefore not be reflected in this document. The final published version may be obtained via the publisher’s DOI. Please note that additional copyright and access restrictions may apply to the published version.Publisher DOI
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