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A protocol for the preparation of cryoprecipitate and cryodepleted plasma

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posted on 27.08.2021, 06:44 by RL Sparrow, David GreeningDavid Greening, Richard SimpsonRichard Simpson
Cryoprecipitate is a concentrate of high-molecular-weight plasma proteins that precipitate when frozen plasma is slowly thawed at 1-6°C. The concentrate contains factor VIII (antihemophilic factor), von Willebrand factor (vWF), fibrinogen, factor XIII, fibronectin, and small amounts of other plasma proteins. Clinical-grade preparations of cryoprecipitate are mainly used to treat fibrinogen deficiency caused by acute bleeding or functional abnormalities of the fibrinogen protein. In the past, cryoprecipitate was used to treat von Willebrand disease and hemophilia A (factor VIII deficiency), but the availability of more highly purified coagulation factor concentrates or recombinant protein preparations has superseded the use of cryoprecipitate for these coagulopathies. Cryodepleted plasma ("cryosupernatant") is the plasma supernatant that remains following removal of the cryoprecipitate from frozen-thawed plasma. It contains all the other plasma proteins and clotting factors present in plasma that remain soluble during cold-temperature thawing of the plasma. © 2011 Springer Science+Business Media, LLC.

History

Publication Date

01/01/2011

Book Title

Serum/plasma proteomics: Methods and protocols

Editors

Simpson RJ Greening DW

Publisher

Humana Press

Place of publication

Melbourne

Series

Methods in Molecular Biology

Volume

728

Pagination

8p. (p. 259-266)

ISBN-13

9781617790676

Rights Statement

The Author reserves all moral rights over the deposited text and must be credited if any re-use occurs. Documents deposited in OPAL are the Open Access versions of outputs published elsewhere. Changes resulting from the publishing process may therefore not be reflected in this document. The final published version may be obtained via the publisher’s DOI. Please note that additional copyright and access restrictions may apply to the published version.