A protocol for the preparation of cryoprecipitate and cryo-depleted plasma for proteomic studies
chapterposted on 13.08.2021, 01:42 authored by RL Sparrow, Richard SimpsonRichard Simpson, David GreeningDavid Greening
Cryoprecipitate is a concentrate of high-molecular-weight plasma proteins that precipitate when frozen plasma is slowly thawed at 1–6 °C. The concentrate contains factor VIII (antihemophilic factor), von Willebrand factor (vWF), fibrinogen, factor XIII, fibronectin, and small amounts of other plasma proteins. Clinical grade preparations of cryoprecipitate are mainly used to treat fibrinogen deficiency caused by acute bleeding or functional abnormalities of the fibrinogen protein. In the past, cryoprecipitate was used to treat von Willebrand disease and hemophilia A (factor VIII deficiency), but the availability of more highly purified coagulation factor concentrates or recombinant protein preparations has superseded the use of cryoprecipitate for these coagulopathies. Cryo-depleted plasma (“cryosupernatant”) is the plasma supernatant remaining following removal of the cryoprecipitate from frozen-thawed plasma. It contains all the other plasma proteins and clotting factors present in plasma that remain soluble during cold-temperature thawing of the plasma. This protocol describes the clinical-scale preparation of cryoprecipitate and cryo-depleted plasma for proteomic studies.
Book TitleSerum/plasma proteomics : methods and protocols
EditorsGreening D Simpson R
Place of publicationNew York
SeriesMethods in Molecular Biology
Pagination8p. (p. 23-30)
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Science & TechnologyLife Sciences & BiomedicineBiochemical Research MethodsBiochemistry & Molecular BiologyPlasmaCryoprecipitateAntihemophilic factorFactor VIIICryo-depletedFibrinogenProteomicsCryosupernatantFRESH-FROZEN PLASMAFACTOR-VIIICONCENTRATEYIELDHumansBlood ProteinsBlood Coagulation FactorsFactor XIIIProteomeAnticoagulantsCryopreservationBlood Specimen CollectionDevelopmental Biology