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A protocol for the preparation of cryoprecipitate and cryo-depleted plasma for proteomic studies.pdf (118.29 kB)

A protocol for the preparation of cryoprecipitate and cryo-depleted plasma for proteomic studies

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posted on 13.08.2021, 01:42 by RL Sparrow, Richard SimpsonRichard Simpson, David GreeningDavid Greening
Cryoprecipitate is a concentrate of high-molecular-weight plasma proteins that precipitate when frozen plasma is slowly thawed at 1–6 °C. The concentrate contains factor VIII (antihemophilic factor), von Willebrand factor (vWF), fibrinogen, factor XIII, fibronectin, and small amounts of other plasma proteins. Clinical grade preparations of cryoprecipitate are mainly used to treat fibrinogen deficiency caused by acute bleeding or functional abnormalities of the fibrinogen protein. In the past, cryoprecipitate was used to treat von Willebrand disease and hemophilia A (factor VIII deficiency), but the availability of more highly purified coagulation factor concentrates or recombinant protein preparations has superseded the use of cryoprecipitate for these coagulopathies. Cryo-depleted plasma (“cryosupernatant”) is the plasma supernatant remaining following removal of the cryoprecipitate from frozen-thawed plasma. It contains all the other plasma proteins and clotting factors present in plasma that remain soluble during cold-temperature thawing of the plasma. This protocol describes the clinical-scale preparation of cryoprecipitate and cryo-depleted plasma for proteomic studies.

History

Publication Date

01/01/2017

Book Title

Serum/plasma proteomics : methods and protocols

Editors

Greening D Simpson R

Publisher

Humana Press

Place of publication

New York

Edition

2nd

Series

Methods in Molecular Biology

Volume

1619

Pagination

8p. (p. 23-30)

ISBN-13

9781493970568

Rights Statement

The Author reserves all moral rights over the deposited text and must be credited if any re-use occurs. Documents deposited in OPAL are the Open Access versions of outputs published elsewhere. Changes resulting from the publishing process may therefore not be reflected in this document. The final published version may be obtained via the publisher’s DOI. Please note that additional copyright and access restrictions may apply to the published version.